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Your search keyword '"Pasin, Thiago Machado"' showing total 27 results
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27 results on '"Pasin, Thiago Machado"'

5. Co-Culture of Trichoderma Reesei, Talaromyces Sp. and Aspergillus Spp. Produces A Multi-Enzyme Cocktail for the Hydrolysis of Sugarcane Bagasse Pretreated with Piperonilic Acid (PIP) and Methylenedioxycinnamic Acid (MDCA)

Author

Silva, Yuri Heck da, primary, de Oliveira, Tássio Brito, additional, Lima, Matheus Sanitá, additional, Pasin, Thiago Machado, additional, Scarcella, Ana Silvia de Almeida, additional, Polizeli, Maria de Lourdes Teixeira de Moraes, additional, Martinez, Carlos Alberto, additional, Buckeridge, Marcos Silveira, additional, Santos, Wanderley Dantas dos, additional, and de Lucas, Rosymar Coutinho, additional

Published
2022
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8. Saccharification of different sugarcane bagasse varieties by enzymatic cocktails produced by Mycothermus thermophilus and Trichoderma reesei RP698 cultures in agro-industrial residues

Author

Scarcella, Ana Sílvia de Almeida, primary, Pasin, Thiago Machado, additional, de Oliveira, Tássio Brito, additional, de Lucas, Rosymar Coutinho, additional, Ferreira-Nozawa, Monica Stropa, additional, Freitas, Emanuelle Neiverth de, additional, Vici, Ana Claudia, additional, Buckeridge, Marcos Silveira, additional, Michelin, Michele, additional, and Polizeli, Maria de Lourdes Teixeira de Moraes, additional

Published
2021
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9. Biochemical characterization of an acid‐thermostable glucoamylase from Aspergillus japonicus with potential application in the paper bio‐deinking.

Author

Pasin, Thiago Machado, Betini, Jorge Henrique Almeida, de Lucas, Rosymar Coutinho, and Polizeli, Maria de Lourdes Teixeira de Moraes

Subjects
GLUCOAMYLASE, AMYLASES, ASPERGILLUS, RECYCLED paper, MANUFACTURING processes, CELLULASE
Abstract

Aspergillus species have been highlighted in enzyme production looking for industrial applications, notably, amylases are one of the most interesting enzymes. They are capable of hydrolyzing α‐glycosidic linkages of starch and widely used in industrial processes to produce ethanol, glucose, and fructose syrup as well as in the textiles, detergents, and paper industries applications. In this context, this work aimed at the biochemical characterization of the glucoamylase from Aspergillus japonicus and its application in the bio‐bleaching process of recycled paper. The optimum temperature and pH for the glucoamylase assay were standardized as 50°C and 5.5. After 1 h of incubation, glucoamylase retained 90% of its activity at 30–50°C. It also kept 70% of its activity in the pH range of 4.0–6.5 after an hour of incubation. The enzyme led to an increase of 30% in the relative whiteness of 10 dry grams of sulfite paper and magazine paper when applied along with commercial cellulase and 10 mM MnCl2. In addition, after the treatments, the glucoamylase recovered activity was 30%–32%, which indicates a prolonged availability of the enzyme and can considerably curtail the redundant downstream process of the recycled paper bio‐bleaching. Thus, the glucoamylase from A. japonicus has a significant role in bio‐bleaching recycled paper, reducing the necessity of hard chemicals, and improving the industrial process in an interesting economic and ecological mode. [ABSTRACT FROM AUTHOR]

Published
2024
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11. The profile secretion of Aspergillus clavatus: Different pre-treatments of sugarcane bagasse distinctly induces holocellulases for the lignocellulosic biomass conversion into sugar

Author

de Lucas, Rosymar Coutinho, primary, de Oliveira, Tássio Brito, additional, Lima, Matheus Sanitá, additional, Pasin, Thiago Machado, additional, Scarcella, Ana Sílvia de Almeida, additional, Ribeiro, Liliane Fraga Costa, additional, Carvalho, Caio, additional, Damasio, André Ricardo de Lima, additional, Buckeridge, Marcos Silveira, additional, Prade, Rolf Alexander, additional, Segato, Fernando, additional, and Polizeli, Maria de Lourdes Teixeira de Moraes, additional

Published
2021
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12. Holocellulase production by filamentous fungi: potential in the hydrolysis of energy cane and other sugarcane varieties

Author

Scarcella, Ana Sílvia de Almeida, primary, Pasin, Thiago Machado, additional, de Lucas, Rosymar Coutinho, additional, Ferreira-Nozawa, Monica Stropa, additional, de Oliveira, Tássio Brito, additional, Contato, Alex Graça, additional, Grandis, Adriana, additional, Buckeridge, Marcos Silveira, additional, and Polizeli, Maria de Lourdes Teixeira de Moraes, additional

Published
2021
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18. Effect of enzymatic pretreatment of sugarcane bagasse with recombinant hemicellulases and esterase prior to the application of the cellobiohydrolase CBH I Megazyme®

Author

de Lucas, Rosymar Coutinho, primary, de Oliveira, Tássio Brito, additional, Lima, Matheus Sanitá, additional, Pasin, Thiago Machado, additional, de Almeida Scarcella, Ana Sílvia, additional, Prade, Rolf Alexander, additional, Segato, Fernando, additional, and de Moraes Polizeli, Maria de Lourdes Teixeira, additional

Published
2020
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26. A novel glucoamylase activated by manganese and calcium produced in submerged fermentation by Aspergillus phoenicis.

Author

Benassi, Vivian Machado, Pasin, Thiago Machado, Facchini, Fernanda Dell Antonio, Jorge, João Atílio, and de Lourdes Teixeira de Moraes Polizeli, Maria

Subjects
ASPERGILLUS, MICROBIOLOGICAL synthesis, MONILIACEAE, MONOSACCHARIDES, THIN layer chromatography
Abstract

This study investigates the production of glucoamylase from Aspergillus phoenicis in Machado Benassi (MB) medium using 1% maltose as carbon source. The maximum amylase activity was observed after four days of cultivation, on static conditions at 30 °C. Glucoamylase production was induced by maltose and inhibited by different glucose concentrations. The optimum of temperature and pH were 60-65 °C, and 4.5 or 5.0 to sodium acetate and Mcllvaine buffers, respectively. It was observed that the enzyme was totally stable at 30-65 °C for 1 h, and the pH range was 3.0-6.0. The enzyme was mainly activated by manganese (176%), and calcium (130%) ions. The products of starch hydrolysis were analyzed by thin layer chromatography and after 3 h, only glucose was detected, characterizing the amylolytic activity as a glucoamylase. [ABSTRACT FROM AUTHOR]

Published
2014
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27. Fungal communities differentially respond to warming and drought in tropical grassland soil.

Author

de Oliveira TB, de Lucas RC, Scarcella ASA, Contato AG, Pasin TM, Martinez CA, and Polizeli MLTM

Subjects
Brazil, Climate Change, Droughts, Grassland, Phylogeny, Soil Microbiology, Mycobiome genetics, Soil
Abstract

Climate change is predicted to cause more extreme events, such as heatwaves, and different precipitation patterns. The effects of warming and short-term drought on soil microbial communities, in particular fungal communities, remain largely unexplored under field conditions. Here, we evaluated how the fungal community of a tropical grassland soil responds to these changes. A field experiment was carried out in a temperature free-air controlled enhancement (T-FACE) facility in Ribeirão Preto, Brazil. The isolated and combined effects of drought and a 2°C increase in temperature were investigated. Based on metabarcoding of the ITS2 region, a total of 771 operational taxonomic units were observed. While warming affected the community structure, drought affected the alpha diversity, and the interaction between warming and drought affected both diversity and structure. The change in community composition driven by warming affected only the less abundant species (>1% of the total sequences). The aspect of the fungal communities that was most affected was diversity, which was increased by drought (p < .05), mostly by reducing the dominance of a single species, as observed in the watered plots. In a phylogenetic context, some fungal taxa were favoured by changes in temperature (Hypocreales) and drought (Sordariales) or disadvantaged by both (Pleosporales). It was of note that a water deficit increased the abundance of phytopathogenic fungi, such as Curvularia, Thielavia and Fusarium species. Overall, our results provide evidence that fungal communities in tropical grassland soils have greater sensitivity to drought than to temperature, which might increase the incidence of certain soil-borne diseases., (© 2020 John Wiley & Sons Ltd.)

Published
2020
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